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rabbit polyclonal anti-mitf  (Millipore)


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    Structured Review

    Millipore rabbit polyclonal anti-mitf
    Rabbit Polyclonal Anti Mitf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+mitf/anti+mitf/pm40138313-256-2-9
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-mitf - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    Recombinant:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    MTT Assay:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    Membrane:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    Plasmid Preparation:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article

    Construct:

    Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
    Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).

    Article Title: Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
    Article Snippet: Anti-phospho(Ser180/73)-MITF rabbit polyclonal antibody (Sigma-Aldrich) was used to detect nuclear phospho-MITF by Western blot.

    Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018

    Article Title: Antagonistic roles for MITF and TFE3 in melanoma plasticity.
    Article Snippet: Article



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    OriGene mitf
    RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated <t>STAT3</t> <t>(Y705),</t> total STAT3, <t>MITF,</t> SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).
    Mitf, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Journal: Cell reports

    Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

    doi: 10.1016/j.celrep.2023.113282

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal Anti-MiTF antibody , Abcam , Cat# ab122982; RRID:AB_10902226.

    Techniques: Recombinant, Plasmid Preparation, Software

    Mean number of cells per millimeter for each marker

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: Mean number of cells per millimeter for each marker

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques:

    A subpopulation of immature melanocytes exists in the interfollicular epidermis that differentiates temporarily during radiation exposure Mean number of cells per millimeter in the basal layer (n = 15 patients). (A) MITF-positive melanocytes ( ) and Bcl-2-positive melanocytes ( ). (B) MITF-negative cells ( ) and Bcl-2-negative cells ( ) are morphologically characterized as melanocytes. Error bars represent SEM.

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: A subpopulation of immature melanocytes exists in the interfollicular epidermis that differentiates temporarily during radiation exposure Mean number of cells per millimeter in the basal layer (n = 15 patients). (A) MITF-positive melanocytes ( ) and Bcl-2-positive melanocytes ( ). (B) MITF-negative cells ( ) and Bcl-2-negative cells ( ) are morphologically characterized as melanocytes. Error bars represent SEM.

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques:

    Number of negative epidermal melanocytes per millimeter and percentage of the total number melanocytes counted for each marker

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet: Number of negative epidermal melanocytes per millimeter and percentage of the total number melanocytes counted for each marker

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques: Control

    Journal: iScience

    Article Title: Human cutaneous interfollicular melanocytes differentiate temporarily under genotoxic stress

    doi: 10.1016/j.isci.2022.105238

    Figure Lengend Snippet:

    Article Snippet: rabbit polyclonal MITF (1:50, IF) , Atlas Antibodies , Cat# HPA003259; RRID: AB_1079381.

    Techniques: Recombinant, Plasmid Preparation, Software

    RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).

    Journal: Biomedicines

    Article Title: RICTOR Affects Melanoma Tumorigenesis and Its Resistance to Targeted Therapy

    doi: 10.3390/biomedicines9101498

    Figure Lengend Snippet: RICTOR-mediated melanoma spheroid growth induces an MIC phenotype ( A , B ) Colo829, A375, and SkMel5 cell lines were cultivated under the same condition as and treated with DMSO or an mTOR inhibitor (OSI-027 1 μM) for 10 days and counted (scale bar represents 1000 μm). Graphs represent the mean ± s.d. of an experiment in triplicate (similar results were obtained in three independent experiments). Significance was calculated using the Student’s t -test: * p < 0.05, ** p < 0.01. ( C ) The expressions of Myc-tagged RICTOR, RICTOR, phosphorylated STAT3 (Y705), total STAT3, MITF, SOX10, and AXL were assessed by Western blotting in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR). ( D ) Relative mRNA expressions of the different tumor-initiating cell markers (JARID, Tie1, and BMPR1), as well as RICTOR, were assessed by qPCR and normalized to GAPDH in Colo829 cells transfected with an empty vector (Control) or with a vector expressing human RICTOR cDNA (RICTOR).

    Article Snippet: Membranes were probed with the following primary antibodies: Myc-Tag, RICTOR, ACTIN, p-AKT (S473), AKT, p-ERK, ERK, pSTAT3 (Y705), STAT3, SOX10, AXL (Cell Signaling Technology, Danvers, MA, USA), and MITF (OriGene Technologies Inc, Rockville, MD, USA).

    Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Expressing