Virus:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
Recombinant:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
MTT Assay:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
Membrane:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
Plasmid Preparation:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
Construct:Article Title: Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure
Article Snippet: Indirect immunofluorescence was performed with the following antibodies: rabbit polyclonal anti-ZNF503 (Sigma-Aldrich, St. Louis, MO, USA; HPA026848, 1:1000), rabbit polyclonal anti-Pax6 (Covance, Princeton, NJ, USA; 1:1000), rabbit polyclonal anti-Pax2 (Invitrogen, Carlsbad, CA, USA; 1:500), goat polyclonal anti-OTX2 (R&D Systems, Minneapolis, MN, USA; AF1979, 1:2000), goat polyclonal anti-Chx10/Vsx2 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA; SC-21690, 1:2000), rabbit polyclonal anti-MITF (Sigma-Aldrich; HPA003259, 1:500), rabbit anti-Mitf (gift from K. Bharti), and Isolectin GS-IB4–Alexa Fluor–488 (Thermofisher Scientific, Carlsbad, CA, USA; I21411, 1:750).
Article Title: Toward Minimal Residual Disease-Directed Therapy in Melanoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-RXRG Abcam Cat#ab15518 Rabbit polyclonal anti-pERK Cell Signaling Cat#4370 Rabbit polyclonal anti-NGFR Cell Signaling Cat#8238 Rabbit polyclonal anti-S100 DAKO Cat#Z0311 Rabbit polyclonal anti-AQP1 Millipore Cat#AB2219 Goat polyclonal anti-AXL R&D Cat#AF154 Goat polyclonal anti-GFRA2 R&D Cat#AF429 Goat polyclonal anti-MITF R&D Cat#AF5769 Goat polyclonal anti-SOX10 Santa Cruz Cat#sc-17342 Rabbit polyclonal anti-CD36 Sigma-Aldrich Cat#HPA002018 Rabbit polyclonal anti-MITF Sigma-Aldrich Cat#HPA003259 Rabbit polyclonal anti-MEF2C Sigma-Aldrich Cat#HPA005533 Rabbit polyclonal anti-TFAP2B Sigma-Aldrich Cat#HPA034683 Rabbit polyclonal anti-MLANA Sigma-Aldrich Cat#HPA048662 Donkey anti-goat Alexa Fluor 594 Thermo Fisher Scientific Cat#A-11058 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat#A-21206 Rabbit polyclonal anti-Ki67 Thermo Fisher Scientific Cat#RM-9106-S0 Donkey Anti-Rabbit IgG Unconjugated Jackson Immunoresearch Cat#711-007-003 Donkey anti-rabbit Fab fragment Alexa Fluor 488 Jackson Immunoresearch Cat#111-547-003 Donkey anti-rabbit Fab fragment Alexa Fluor 594 Jackson Immunoresearch Cat#711-587-003 Bacterial and Virus Strains dsRed encoding viral vector, pHR’SINcPPTSGW RFP (VSV-G typed lentivirus, packed with third generation packaging constructs) Laboratory for Translational Research in GastroIntestinal Disorders N/A Biological Samples Patient biopsies Massachusetts General Hospital https://www.massgeneral.org/ Patient biopsies University of Zürich Hospital http://www.en.usz.ch/Pages/default.aspx Patient-derived xenografts (PDXs) KU Leuven https://www.uzleuven-kuleuven.be/lki/trace/ Chemicals, Peptides, and Recombinant Proteins Agencourt AMPure XP beads Agencourt/ Beckman coulter Cat#A63881 Bexarotene MCE Cat#HY-14171 Dabrafenib MCE Cat#HY-14660 Trametinib MCE Cat#HY-10999 Normal Donkey Serum Jackson Immuneresearch Cat#017-000-121 Collagenase Type I Sigma-Aldrich Cat#C0130 Collagenase Type IV Sigma-Aldrich Cat#C5138 KAPA HiFi Hot start Readymix Sopachem Cat#KK2602 Superscript II Thermo Fisher Scientific Cat#10328062 SYTOX Blue Thermo Fisher Scientific Cat#S34857 Trypsin (0.25%) Thermo Fisher Scientific Cat#25200056 DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) Thermo Fisher Scientific Cat#D3571 HX531 Tocris Cat#3912 F-10 Nut Mix (Ham) GIBCO Cat#22390-025 (Continued on next page) e1 Cell 174, 1–13.e1–e8, August 9, 2018
|